■基礎研究から学ぶ
2.組織細胞化学シリーズ(若手研究者へのヒント)光学免疫組織化学の基礎:固定と凍結切片を用いた蛍光免疫組織化学(1)
瀧澤 俊広
日本医科大学大学院医学研究科分子解剖学
2. Histocytochemistry Series Light Microscopic Immunohistochemistry: Fixation and Immunofluorescence Microscopy Using Cryostat-sections (1)
Toshihiro Takizawa
Department of Molecular Anatomy and Medicine, Nippon Medical School

Histochemistry, especially immunohistochemistry, is a powerful and diverse set of methods for obtaining spatial and temporal information concerning the expression and distribution of biomolecules in situ. In this technical note, we describe procedures for fixation and immunofluorescence microscopy using cryostat sections.
日医大医会誌 2009; 5(2), 136-140
Key words
fixation, immunohistochemistry, immunofluorescence microscopy
Correspondence to
Toshihiro Takizawa, Department of Molecular Anatomy and Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
E-mail:t-takizawa@nms.ac.jp
受付:2009年3月4日 受理:2009年3月18日 |