■基礎研究から学ぶ
2．組織細胞化学シリーズ（若手研究者へのヒント） 共焦点レーザー顕微鏡による形態，機能解析―マルチモード顕微鏡による癌細胞の形態，機能解析の実際（2）
内藤 善哉
日本医科大学大学院医学研究科統御機構病理学
2. Histocytochemistry Series Morphological and Functional Analyses of Cells, Using Confocal Laser Scanning Microscopy System: Example for Morphological and Functional Analyses of Cancer Cells, Using Multi-mode Microscopy System (2)
Zenya Naito
Department of Integrative Pathology, Graduate School of Medicine, Nippon Medical School
The confocal laser scanning microscope (CLSM) is a device for obtaining high-resolution optical images of immunofluorescent staining. The CLSM can produce in-focus images of thick specimens, a process known as optical sectioning. The images are reconstructed with a computer, using 3-dimensional image software, allowing 3-dimensional reconstructions of topologically complex objects. On the same tissue sections, the CLSM can obtain the images of differential interference contrast. Recently, a special inverted CLSM -the multimode microscopy system- has been used to examine the morphology and functions of cells. A multimode microscopy system can be used to obtain images of CLSM, total internal reflection fluorescence, time-lapse, and micromanipulation. In the present study, we show images of pancreatic cancer cells as an example.
日医大医会誌 2009; 5(3), 159-166
Key words
confocal laser scanning microscopy, immunofluorescence, differential interference contrast, three-dimensional image, cancer
Correspondence to
Dr Zenya Naito, Department of Pathology, Integrative Oncological Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku Tokyo 113-8602, Japan
E-mail：naito@nms.ac.jp
受付：2009年4月6日 受理：2009年4月16日 |
全文PDF