第5巻 2009年10月　第4号

■基礎研究から学ぶ

2．組織細胞化学シリーズ（若手研究者へのヒント）　免疫電子顕微鏡法の基礎（3）
小澤　一史, 松崎　利行
日本医科大学大学院医学研究科生体制御形態科学

2. Histocytochemistry Series The Fundamentals of Immunoelectron Microscopy (3)
Hitoshi Ozawa and Toshiyuki Matsuzaki
Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School

Immunohistochemistry is concerned with the detection of specific biological substances at the light and electron microscopic levels with antibodies labeled with visible markers, such as horseradish peroxidase and colloidal gold. In particular, the immunohistochemistry of electron microscopy has provided much morphological and biological information. Immunoelectron microscopy can be classified into three methods, i.e., pre-embedding, postembedding, and nonembedding methods, on the basis of the step during which the immunoreaction is applied to the biological specimens. Each method has both advantages and disadvantages, so we should select the method according to the biological purpose. An overview of immunoelectron microscopy is given, and several electron micrographs using immunohistochemical techniques are shown.

日医大医会誌 2009; 5(4), 215-220

Key words
immunoelectron microscopy, pre-embedding method, nanogold method, post-embedding method, non-embedding method

Correspondence to
Hitoshi Ozawa, Department of Anatomy and Neurobiology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku Tokyo 113-8602, Japan
E-mail：hozawa@nms.ac.jp

受付：2009年8月14日　受理：2009年9月9日