■基礎研究から学ぶ
2.組織細胞科学シリーズ（若手研究者へのヒント） 病理標本における免疫組織化学の基礎（5）
清水 章, 若松 恭子, 益田 幸成, 福田 悠
日本医科大学大学院医学研究科解析人体病理学
2. Histocytochemistry Series Immunohistochemistry in Pathology (5)
Akira Shimizu, Kyoko Wakamatsu, Yukinari Masuda and Yuh Fukuda
Department of Analytic Human Pathology, Graduate School of Medicine, Nippon Medical School
Immunohistochemistry is a technique to examine the expression and distribution of biomolecules in situ using antibodies labeled with enzymes, such as horseradish peroxidase. Generally, both immunohistochemistry and immunofluorescence are termed as immunostaining, because localized biomolecules in situ are detected with antigen-antibody immunoreactions producing color signals. Immunostaining techniques are divided into 3 types: direct, indirect, and amplification methods (peroxidase-antiperoxidase, avidin-biotin complex, and polymer). Because each method has advantages and disadvantages, the method should be selected according to the biological purpose. In this technical note, we describe mechanisms and procedures for immunostaining, including immunohistochemistry and immunofluorescence.
日医大医会誌 2010; 6(3), 140-146
Key words
immunohistochemistry, immunofluorescence, immunostaining, double staining
Correspondence to
Akira Shimizu, Department of Analytic Human Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
E-mail：ashimizu@nms.ac.jp
受付：2010年4月28日 受理：2010年5月21日 |
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