第6巻 2010年10月　第4号

■基礎研究から学ぶ

2．組織細胞化学シリーズ（若手研究者へのヒント）　最近の免疫組織化学賦活法（6）
並松　茂樹, 杉崎　祐一, 土屋　眞一
日本医科大学付属病院病理部

2. Histocytochemistry Series A New Antigen Retrieval Method (6)
Shigeki Namimatsu, Yuichi Sugisaki and Shinichi Tsuchiya
Division of Diagnostic Pathology, Nippon Medical School Hospital

Current antigen retrieval techniques include the use of citrate buffer, Tris-HCl containing 5% urea, and EDTA solutions combined with heating in a microwave oven or autoclave. These methods must be adjusted for a given tissue or antigen. To improve the efficiency of antigen retrieval for immunohistochemical staining, we developed a new method using citraconic anhydride. We describe this new antigen retrieval method using 0.05% citraconic anhydride solution of pH 7.4 and heat. This antigen retrieval method produced satisfactory staining results for a wide variety of antigens.

日医大医会誌 2010; 6(4), 178-184

Key words
antigen retrieval, citraconic anhydride, immunohistochemistry

Correspondence to
Shigeki Namimatsu, Division of Diagnostic Pathology, Nippon Medical School Hospital, 1-1-5 Sendagi, Bunkyo-ku Tokyo 113-8603, Japan
E-mail：namimatu@nms.ac.jp

受付：2010年8月20日　受理：2010年9月9日