■綜説
法医中毒学における質量分析研究の新展開
林田 眞喜子, 大野 曜吉
日本医科大学大学院医学研究科法医学
New Developments in Mass Chromatography-spectrometry Research for Forensic Toxicology
Makiko Hayashida and Youkichi Ohno
Department of Legal Medicine, Graduate School of Medicine, Nippon Medical School
Toxicological analysis with mass chromatography-spectrometry has spread widely and quickly with the progress in ionizing methods.
This review describes new developments in mass chromatography-spectrometry research for forensic toxicology. Time-of-flight (TOF) mass spectrometry (MS) coupled with liquid chromatography (LC) has been developed for screening and for determining benzodiazepines with an exact mass database. All benzodiazepines have similar chemical structures and molecular weights, and, thus, show similar mass spectra and protonated molecule ions. Discrimination of mass spectrometry at low resolving power with LC-MS is usually difficult.
Benzodiazepines are widely used in medical practice in Japan, and data acquired from LC/TOF-MS measurements of 130 drugs, including benzodiazepines, were used to create an exact mass database. This database comprises molecular formulae, calculated exact masses, retention times and calibrations. In the analysis of samples from patients who had taken many drugs, selectivity was improved when the LC/TOF-MS exact mass database was used.
Comprehensive screening with LC/quadrupole TOF is the most effective method in forensic toxicology for analyzing the samples from cases of multidrug overdose. New developmental mass chromatography-spectrometry equipment is required because of the more precise and exact analyses in forensic toxicology.
日医大医会誌 2011; 7(1), 8-15
Key words
forensic toxicology, liquid chromatography/mass spectrometry, screening, benzodiazepines, serum
Correspondence to
Makiko Hayashida, Department of Legal Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku Tokyo 113-8602, Japan
E-mail：mh@nms.ac.jp
受付：2010年8月30日 受理：2010年11月5日 |
全文PDF