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Abstract

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3. Gene Delivery and Expression Series Gene Delivery and Expression (1)
Hideo Orimo
Division of Medical and Biological Chemistry, Graduate School of Medicine, Nippon Medical School

The delivery of genes into cells is a basic procedure in molecular biology. The induction of genes into prokaryotic cells is referred to as transformation, which is a basic method in gene cloning, whereas that into eukaryotic cells is called transfection. Several transfection methods have been reported: physical, chemical, and biological. In this technical note, a transfection method using cationic lipids is described for studies of gene function and the expression of mutant proteins.

“ϊˆγ‘εˆγ‰οŽ 2011; 7(2), 92-96

Key words
transformation, transfection, vector, cationic lipids, luciferase

Correspondence to
Hideo Orimo, MD, PhD, Division of Metabolism and Nutrition, Department of Biochemistry and Molecular Biology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku Tokyo 113-8602, Japan
E-mailForimohd@nms.ac.jp

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