第7巻 2011年4月　第2号

■基礎研究から学ぶ

3．遺伝子導入と発現シリーズ　遺伝子導入と発現（1）
折茂　英生
日本医科大学大学院医学研究科医科生物化学

3. Gene Delivery and Expression Series Gene Delivery and Expression (1)
Hideo Orimo
Division of Medical and Biological Chemistry, Graduate School of Medicine, Nippon Medical School

The delivery of genes into cells is a basic procedure in molecular biology. The induction of genes into prokaryotic cells is referred to as transformation, which is a basic method in gene cloning, whereas that into eukaryotic cells is called transfection. Several transfection methods have been reported: physical, chemical, and biological. In this technical note, a transfection method using cationic lipids is described for studies of gene function and the expression of mutant proteins.

日医大医会誌 2011; 7(2), 92-96

Key words
transformation, transfection, vector, cationic lipids, luciferase

Correspondence to
Hideo Orimo, MD, PhD, Division of Metabolism and Nutrition, Department of Biochemistry and Molecular Biology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku Tokyo 113-8602, Japan
E-mail：orimohd@nms.ac.jp

受付：2011年2月21日　受理：2011年3月30日