第7巻 2011年10月　第4号

■基礎研究から学ぶ

3．遺伝子導入と発現シリーズ　バクテリアを利用した遺伝子導入とタンパク質の大量発現（2）
松村　智裕
日本医科大学大学院医学研究科医科生物化学

3. Gene Delivery and Expression Series Overexpression of Recombinant Protein in Bacterial Cells (2)
Tomohiro Matsumura
Division of Medical and Biological Chemistry, Graduate School of Medicine, Nippon Medical School

The protein expression system in bacteria is widely used to overproduce recombinant proteins. Many established expression vectors are on the market. The Escherichia coli expression system is extremely useful for biochemical and biophysical analysis. Here, we describe the results of the expression of mammalian protein in E. coli and an experiment on the structural analysis of recombinant protein.

日医大医会誌 2011; 7(4), 169-174

Key words
expression vector, Escherichia coli, overexpression

Correspondence to
Tomohiro Matsumura, Division of Metabolism and Nutrition, Department of Biochemistry and Molecular Biology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
E-mail：tm28@nms.ac.jp

受付：2011年9月1日　受理：2011年9月7日