“ϊ–{ˆγ‰Θ‘εŠwˆγŠw‰οŽGŽ Online Journal ƒƒCƒ“ƒiƒrƒQ[ƒVƒ‡ƒ“‚π”ς‚Ξ‚·
‘–ΪŽŸ > †–ΪŽŸ > Abstract ƒz[ƒ€‚Φ–ί‚ι
Abstract

‘ζ7Šͺ 2011”N10ŒŽ@‘ζ4†

‘S•ΆPDF (507K)

‘Šξ‘bŒ€‹†‚©‚ηŠw‚Τ

3Dˆβ“`Žq“±“ό‚Ζ”­Œ»ƒVƒŠ[ƒY@ƒoƒNƒeƒŠƒA‚π—˜—p‚΅‚½ˆβ“`Žq“±“ό‚Ζƒ^ƒ“ƒpƒNŽΏ‚Μ‘ε—Κ”­Œ»i2j
Ό‘Ί@’q—T
“ϊ–{ˆγ‰Θ‘εŠw‘εŠw‰@ˆγŠwŒ€‹†‰Θˆγ‰ΘΆ•¨‰»Šw

3. Gene Delivery and Expression Series Overexpression of Recombinant Protein in Bacterial Cells (2)
Tomohiro Matsumura
Division of Medical and Biological Chemistry, Graduate School of Medicine, Nippon Medical School

The protein expression system in bacteria is widely used to overproduce recombinant proteins. Many established expression vectors are on the market. The Escherichia coli expression system is extremely useful for biochemical and biophysical analysis. Here, we describe the results of the expression of mammalian protein in E. coli and an experiment on the structural analysis of recombinant protein.

“ϊˆγ‘εˆγ‰οŽ 2011; 7(4), 169-174

Key words
expression vector, Escherichia coli, overexpression

Correspondence to
Tomohiro Matsumura, Division of Metabolism and Nutrition, Department of Biochemistry and Molecular Biology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
E-mailFtm28@nms.ac.jp

Žσ•tF2011”N9ŒŽ1“ϊ@Žσ—F2011”N9ŒŽ7“ϊ

ƒƒCƒ“ƒiƒrƒQ[ƒVƒ‡ƒ“‚Φ–ί‚ι ‚±‚Μƒy[ƒW‚Μƒgƒbƒv‚Φ–ί‚ι