3. Gene Delivery and Expression Series Overexpression of Recombinant Protein in Bacterial Cells (2)
Division of Medical and Biological Chemistry, Graduate School of Medicine, Nippon Medical School
The protein expression system in bacteria is widely used to overproduce recombinant proteins. Many established expression vectors are on the market. The Escherichia coli expression system is extremely useful for biochemical and biophysical analysis. Here, we describe the results of the expression of mammalian protein in E. coli and an experiment on the structural analysis of recombinant protein.
ϊγεγο 2011; 7(4), 169-174
expression vector, Escherichia coli, overexpression
Tomohiro Matsumura, Division of Metabolism and Nutrition, Department of Biochemistry and Molecular Biology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan