■原著
アレルギー性接触皮膚炎の感作相は少なくとも2つのステップより構成され，その完成は皮膚の抗原に対する反応性の向上に重要である
金森 幸男1, 金子 勝美1, 渡理 英二2, 川名 誠司1
1日本医科大学皮膚科学
2日本医科大学微生物学・免疫学
The Sensitization Phase of Allergic Contact Dermatitis Consists of at Least 2 Steps and Its Completion Plays a Crucial Role in the Response to All Antigens
Sachio Kanamori1, Katsumi Kaneko1, Eiji Watari2 and Seiji Kawana1
1)Department of Dermatology, Nippon Medical School
2)Department of Microbiology and Immunology, Nippon Medical School
We examined whether 0.1% 2,4-Dinitorofluorobenzene (DNFB), which is a concentration insufficient to induce contact dermatitis with a single application, can induce dermatitis with repeated applications. 0.1% DNFB was painted on the shaved backs of C57BL/6 mice 2 consecutive days a week and 6 applications (3 weeks) induced allergic contact dermatitis. A hapten-specific lymphocyte proliferative response was observed in regional lymph node cells obtained 2 applictions (1 week) of 0.1% DNFB. Four applications (2 weeks) of 0.1% DNFB did not elicit dermatitis, despite the appearance of hapten-specific lymphocytes; therefore, immunological memory is not sufficient to complete the sensitization phase. Interferon (IFN)-γ was produced at 1 week and in significantly higher amounts at 2 weeks. The redox status of regional lymph node cells was examined, because reductive cells have been reported to produce IFN-γ. The number of reductive cells was also increased at 1 week and significantly higher at 2 weeks. Most of the increased reductive cells were considered to be antigen non-specific because even repeated immunization can not induce such a high number of antigen specific cells. If the higher number of antigen non-specific reductive cells play a crucial role in completing the sensitization phase, even irrelevant antigens could induce dermatitis after 4 applications (2 weeks) of DNFB. Although 2.5% or 3.5% oxazolone could not induce dermatitis with a single application, it could induce dermatitis, if applied following 4 applications (2 weeks) of 0.1% DNFB. These results demonstrate that the sensitization phase of allergic contact dermatitis consists of at least 2 steps: the first step, which is antigen specific, is the appearance of antigen specific lymphocytes in regional lymph nodes, and the second step, which is antigen non-specific, is increases in IFN-γ production and the number of reductive cells. Entry of an antigen to the skin plays a crucial role in the response to all antigens.
日医大医会誌 2012; 8(3), 199-206
Key words
sensitization phase, antigen specific step, antigen non-specific step, susceptibility
Correspondence to
Sachio Kanamori, Department of Dermatology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku Tokyo 113-8603, Japan
E-mail：kanamori@nms.ac.jp
受付：2011年8月15日 受理：2012年2月28日 |
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