■綜説
整形外科学領域における逆転写―定量PCR（RT-qPCR）法で使用する安定な参照遺伝子選択の重要性
渡部 寛1, 2, 石井 寛高2, 大島 康史1, 高井 信朗1, 小澤 一史2
1日本医科大学大学院医学研究科整形外科学分野
2日本医科大学大学院医学研究科解剖学・神経生物学分野
Stable Reference Gene Selection for Reverse Transcription-Quantitative PCR (RT-qPCR) Analyses in Orthopaedic Research
Hiroshi Watanabe1, 2, Hirotaka Ishii2, Yasushi Oshima1, Shinro Takai1 and Hitoshi Ozawa2
1)Department of Orthopaedic Surgery, Nippon Medical School
2)Department of Anatomy and Neurobiology, Nippon Medical School
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a simple and effective method to quantify RNA molecules. This technique has become an essential tool to determine gene expression profiles in target cells and tissues. Although RT-qPCR is highly sensitive and specific, it requires appropriate selection of optimal reference genes for normalization to assure accurate and reliable results. In our recent study, we assessed expression stability of the candidate reference genes for normalization in the synovium of knee osteoarthritis and evaluated the impacts of the reference gene choice on gene expression profiles. In the present manuscript, we will introduce our recent article, explain the way to select suitable reference genes for normalization of RT-qPCR data, and review the suitable reference genes for RT-qPCR analyses in orthopaedic research.
日医大医会誌 2019; 15(1), 24-31
Key words
normalization, orthopaedic surgery, osteoarthritis, reference gene, synovium
Correspondence to
Hirotaka Ishii, Department of Anatomy and Neurobiology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
E-mail：hirotaka@nms.ac.jp
受付：2018年8月31日 受理：2018年10月25日 |
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