第19巻 2023年12月　第4号

■綜説

特異的抗ヒトERβモノクローナル抗体PPZ0506発見がERβ研究に与えた衝撃
石井　寛高, 服部　裕次郎, 肥後　心平, 森下　雅大, 小澤　実那, 大塚　真衣, 松本　恵介, 小澤　一史^†
日本医科大学大学院医学研究科解剖学・神経生物学
^†佛教大学保健医療技術学部

Impact of Well-validated Anti-human ERβ Monoclonal Antibody PPZ0506 on ERβ Research
Hirotaka Ishii, Yujiro Hattori, Shimpei Higo, Masahiro Morishita, Mina Ozawa, Mai Otsuka, Keisuke Matsumoto and Hitoshi Ozawa^†
Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School
^†School of Health Sciences, Bukkyo University

Two subtypes of estrogen receptors (ERs) have been identified in mammals: ERα and ERβ. Because effective antibodies against ERα proteins are available, expression and localization profiles of ERα proteins have been fully determined. By contrast, the paucity of well-validated antibodies against ERβ proteins has caused confusion regarding their expression and localization profiles, which has severely hindered the progress of ERβ research. Notably, the recent discovery of a monoclonal antibody (PPZ0506) specific for human ERβ proteins and its cross-reactivity to rat and mouse ERβ proteins has stimulated development of ERβ detection systems and the use of these systems to analyze the true localization profiles of ERβ proteins. In our previous studies, we reported the development and optimization of immunohistochemical staining methods for rat and mouse ERβ proteins with PPZ0506. Our immunohistochemical results revealed that rat and mouse ERβ proteins are expressed only in more localized tissues and cells than previously assumed, and further indicated that considerable species differences exist in ERβ expression among humans, rats, and mice. In the present review, we discuss various problems in previous ERβ research, new findings obtained using the PPZ0506 antibody, and prospects for future ERβ research.

日医大医会誌 2023; 19(4), 332-338

Key words
antibody validation, ERβ, ESR2, immunohistochemistry, PPZ0506

Correspondence to
Hirotaka Ishii, Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
E-mail：hirotaka@nms.ac.jp

受付：2022年12月6日　受理：2022年12月22日