Journal of Nippon Medical School: Vol.66 No.1 page.015

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ArticleTitle Effects of temperature and preservation time on the pharmacological response of isolated vascular endothelial and smooth muscle function

AuthorList Hideyuki Iwaki¹⁾, Atsuhiro Sakamoto²⁾ and Shigeo Tanaka¹⁾

Affiliation ¹⁾The Second Department of Surgery, ²⁾Department of Anesthesiology, Nippon Medical School

Language EN

Volume 66

Issue 1

Year 1999

Page 15-20

Received May 8, 1998

Accepted October 12, 1998

Keywords cold preservation, temperature, time, endothelium, smooth muscle

Abstract In clinical transplantation and cardiovascular surgery, cold preservation is usually used because it is a simple method. However, the established temperature is by no means exact. The aim of this study was to find the optimum storage temperature for preservation of the vasculature by observing the pharmacological endothelium and smooth muscle response. The thoracic aorta of 36 male Wister rats were studied in organ baths: as fresh control, after 24 hours, 48 hours and 72 hours of storage at 0.5C° , 4C° and 8C° in Krebs-Henseleit bicarbonate (KHB) solution. Acetylcholine (Ach) was used to elicit endothelium-dependent relaxation, and sodium nitroprusside (SNP) to elicit smooth muscle-dependent relaxation. The contractility caused by Phenylephrine (Ph) was influenced by time but before 48 hours it was not influenced by preservation temperature. Significant responsive deterioration by Ach and SNP was seen after 24 hours of storage at 0.5C° as compared with storage at 4C° and 8C° . The endothelium-dependent relaxing function and smooth muscle-dependent relaxing function were best preserved at 4C° and 8C° . These results indicate that precise temperature control is necessary for vessel preservation in clinical situations.

Correspondence to Hideyuki Iwaki, The Second Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan

ArticleTitle	Effects of temperature and preservation time on the pharmacological response of isolated vascular endothelial and smooth muscle function
AuthorList	Hideyuki Iwaki¹⁾, Atsuhiro Sakamoto²⁾ and Shigeo Tanaka¹⁾
Affiliation	¹⁾The Second Department of Surgery, ²⁾Department of Anesthesiology, Nippon Medical School
Language	EN
Volume	66
Issue	1
Year	1999
Page	15-20
Received	May 8, 1998
Accepted	October 12, 1998
Keywords	cold preservation, temperature, time, endothelium, smooth muscle
Abstract	In clinical transplantation and cardiovascular surgery, cold preservation is usually used because it is a simple method. However, the established temperature is by no means exact. The aim of this study was to find the optimum storage temperature for preservation of the vasculature by observing the pharmacological endothelium and smooth muscle response. The thoracic aorta of 36 male Wister rats were studied in organ baths: as fresh control, after 24 hours, 48 hours and 72 hours of storage at 0.5C° , 4C° and 8C° in Krebs-Henseleit bicarbonate (KHB) solution. Acetylcholine (Ach) was used to elicit endothelium-dependent relaxation, and sodium nitroprusside (SNP) to elicit smooth muscle-dependent relaxation. The contractility caused by Phenylephrine (Ph) was influenced by time but before 48 hours it was not influenced by preservation temperature. Significant responsive deterioration by Ach and SNP was seen after 24 hours of storage at 0.5C° as compared with storage at 4C° and 8C° . The endothelium-dependent relaxing function and smooth muscle-dependent relaxing function were best preserved at 4C° and 8C° . These results indicate that precise temperature control is necessary for vessel preservation in clinical situations.
Correspondence to	Hideyuki Iwaki, The Second Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan