Journal of Nippon Medical School: Vol.66 No.2 page.107

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ArticleTitle Examination of non-transmitter effects of catecholamines on clonal cells derived from drosophila CNS

AuthorList Tianxiang Zhang

Affiliation Department of Pharmacology, Nippon Medical School

Language JA

Volume 66

Issue 2

Year 1999

Page 113-118

Received December 10, 1998

Accepted December 28, 1998

Keywords catecholamines, cell proliferation, oxidative product, clonal neuronal cells

Abstract The effects of catecholamines (CAs) other than their transmitter action were investigated using clonal neuronal cells, ML-DmBG2-c2, derived from Drosophila in the larval central nervous system (CNS). All catecholamines tested, adrenaline (AD), dopamine (DA), noradrenaline (NA) and isoproterenol (ISO), prevented any increase in the number of cells during 2-to 7-day culture. α-, β-adrenergic and dopaminergic antagonists did not block the effects of CAs on the number of cells. Adrenochrome, a product of the oxidative degradation of AD, also prevented any increase in the number of cells, as AD did. The effect of AD was partially blocked by an antioxidant, dithiothreitol (DTT). These results suggest that the inhibition of the increase in cell numbers by CA might be mediated by CAs themselves and/or oxidative products in the CA metabolic process. It is concluded that CAs inhibit cell proliferation but do not induce cell death in the Drosophila clonal cells.

Correspondence to Tianxiang Zhang, Department of Pharmacology, Nippon Medical School, Sendagi, Bunkyo-ku, Tokyo 113-8032, Japan

ArticleTitle	Examination of non-transmitter effects of catecholamines on clonal cells derived from drosophila CNS
AuthorList	Tianxiang Zhang
Affiliation	Department of Pharmacology, Nippon Medical School
Language	JA
Volume	66
Issue	2
Year	1999
Page	113-118
Received	December 10, 1998
Accepted	December 28, 1998
Keywords	catecholamines, cell proliferation, oxidative product, clonal neuronal cells
Abstract	The effects of catecholamines (CAs) other than their transmitter action were investigated using clonal neuronal cells, ML-DmBG2-c2, derived from Drosophila in the larval central nervous system (CNS). All catecholamines tested, adrenaline (AD), dopamine (DA), noradrenaline (NA) and isoproterenol (ISO), prevented any increase in the number of cells during 2-to 7-day culture. α-, β-adrenergic and dopaminergic antagonists did not block the effects of CAs on the number of cells. Adrenochrome, a product of the oxidative degradation of AD, also prevented any increase in the number of cells, as AD did. The effect of AD was partially blocked by an antioxidant, dithiothreitol (DTT). These results suggest that the inhibition of the increase in cell numbers by CA might be mediated by CAs themselves and/or oxidative products in the CA metabolic process. It is concluded that CAs inhibit cell proliferation but do not induce cell death in the Drosophila clonal cells.
Correspondence to	Tianxiang Zhang, Department of Pharmacology, Nippon Medical School, Sendagi, Bunkyo-ku, Tokyo 113-8032, Japan