Journal of Nippon Medical School: Vol.67 No.3 page.177

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ArticleTitle Overexpression and Localization of Heat Shock Proteins mRNA in Pancreatic Carcinoma

AuthorList Masao Ogata, Zenya Naito, Shigeo Tanaka,Yuhkichi Moriyama and Goro Asano

Affiliation Department of Pathology, Second Department of Surgery,The Center for Digestive Diseases, Nippon Medical School

Language EN

Volume 67

Issue 3

Year 2000

Page 177-185

Received December 27, 2000

Accepted January 12, 2000

Keywords heat shock proteins, immunohistochemistry, ISH (In situ hybridization), RT -PCR, pancreatic carcinoma

Abstract In the present study we examined the localization and overexpression of heat shock proteins (hsps), mainly hsp90, in pancreatic carcinoma tissue compared with control tissue (including chronic pancreatitis and normal pancreas tissue), with the aid of immunohistochemical staining, in situ hybridization and reverse transcriptase polymerase chain reaction. Hsp90 α mRNA was overexpressed more highly in pancreatic carcinoma than in the control tissue. The proliferating-cell-nuclear-antigen labeling index was also high in pancreatic carcinoma tissue compared with the other tissue. These findings suggest that the overexpression of hsp90 α mRNA in carcinomas may be correlated with cell proliferation. However, hsp90 β was constitutively overexpressed almost equally in all groups of pancreatic tissue including pancreatic carcinoma, chronic pancreatitis and normal pancreas tissue. Immunohistochemical staining demonstrated a differentiation in the expression of hsp90 between histological types of pancreatic carcinoma. These findings suggest that hsp90 α is involved in carcinogenesis and that hsp90 β is correlated to structural conformation. Hsp90 α and hsp90 β seem to perform different functions in tissue containing malignant cells. P53, MDM2 and WAF1, that were cell-cycle-related oncogene product were more strongly expressed in the nuclei of the cancer cells of the cancer tissue. Especially, MDM2 was more strongly expressed in mucinous carcinoma and the mucin secreting tissues surrounding pancreatic carcinoma tissue. The expression of MDM2 protein might also be correlated to secretion systems during structural conformation and be correlated to hsp90 β .

Correspondence to Masao Ogata, Department of Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.
masao-66@dd.iij4u.or.jp

ArticleTitle	Overexpression and Localization of Heat Shock Proteins mRNA in Pancreatic Carcinoma
AuthorList	Masao Ogata, Zenya Naito, Shigeo Tanaka,Yuhkichi Moriyama and Goro Asano
Affiliation	Department of Pathology, Second Department of Surgery,The Center for Digestive Diseases, Nippon Medical School
Language	EN
Volume	67
Issue	3
Year	2000
Page	177-185
Received	December 27, 2000
Accepted	January 12, 2000
Keywords	heat shock proteins, immunohistochemistry, ISH (In situ hybridization), RT -PCR, pancreatic carcinoma
Abstract	In the present study we examined the localization and overexpression of heat shock proteins (hsps), mainly hsp90, in pancreatic carcinoma tissue compared with control tissue (including chronic pancreatitis and normal pancreas tissue), with the aid of immunohistochemical staining, in situ hybridization and reverse transcriptase polymerase chain reaction. Hsp90 α mRNA was overexpressed more highly in pancreatic carcinoma than in the control tissue. The proliferating-cell-nuclear-antigen labeling index was also high in pancreatic carcinoma tissue compared with the other tissue. These findings suggest that the overexpression of hsp90 α mRNA in carcinomas may be correlated with cell proliferation. However, hsp90 β was constitutively overexpressed almost equally in all groups of pancreatic tissue including pancreatic carcinoma, chronic pancreatitis and normal pancreas tissue. Immunohistochemical staining demonstrated a differentiation in the expression of hsp90 between histological types of pancreatic carcinoma. These findings suggest that hsp90 α is involved in carcinogenesis and that hsp90 β is correlated to structural conformation. Hsp90 α and hsp90 β seem to perform different functions in tissue containing malignant cells. P53, MDM2 and WAF1, that were cell-cycle-related oncogene product were more strongly expressed in the nuclei of the cancer cells of the cancer tissue. Especially, MDM2 was more strongly expressed in mucinous carcinoma and the mucin secreting tissues surrounding pancreatic carcinoma tissue. The expression of MDM2 protein might also be correlated to secretion systems during structural conformation and be correlated to hsp90 β .
Correspondence to	Masao Ogata, Department of Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan. masao-66@dd.iij4u.or.jp