Journal of Nippon Medical School: Vol.68 No.5 page.384

Home > List of Issue > Table of Contents > Abstract

Journal of Nippon Medical School

Select Language
in Japanese < > in English

Full Text of this Article

in English PDF (1024k)

ArticleTitle Expression and Localization of Basic Fibroblast Growth Factor and its mRNA in Solitary Fibrous Tumor

AuthorList Xianfeng Li¹, Shotaro Maeda², Masaru Hosone², Hironori Katayama², Namie Sawada¹, Yuliang Sun¹, Toshiyuki Ishiwata¹, Munehiro Yokoyama¹, Zenya Naito¹ and Goro Asano¹

Affiliation ¹Department of Pathology, Nippon Medical School, ²Department of Pathology, Tama Nagayama Hospital, Nippon Medical School

Language EN

Volume 68

Issue 5

Year 2001

Page 384-392

Received March 14, 2001

Accepted April 17, 2001

Keywords solitary fibrous tumor, basic fibroblast growth factor, immunohistochemistry, in situ hybridization

Abstract Solitary fibrous tumors (SFTs) represent a distinct neoplasm that should be included in the differential diagnosis of spindle-cell neoplasms of the soft tissue. Basic fibroblast growth factor (bFGF or FGF-2) is a mitogenic and angiogenic polypeptide produced by diverse cell types, including the cells derived from normal tissue and neoplastic lesions. In this study, the expression of bFGF, vimentin, CD 34, c-kit (or CD 117), desmin, S-100 protein, and α-smooth muscle actin (α-SMA) in SFTs, hemangiopericytomas (HPC), gastrointestinal stromal tumors (GIST), and dermatofibrosarcoma protuberans (DFSP) were evaluated to assess their usefulness in the differential diagnosis of these lesions. The expression of bFGF mRNA was also examined in SFTs by in situ hybridization (ISH) using a digoxigenin-labeled bFGF oligonucleotide probe. All the SFTs, GISTs and DFSPs exhibited strong and diffuse immunoreactivity for CD34 and vimentin, and were completely negative for desmin, S-100 protein and α-SMA. The HPCs were positive for vimentin, but negative for CD34. In all the SFTs, strong and diffuse nuclear immunostaining was observed with bFGF antibody, contrasting with the negative staining observed in the majority of the HPCs, GISTs, and DFSPs. The bFGF mRNA was also expressed in the SFT cells. The constitutive expression of the bFGF in the SFT widens the spectrum of available markers for these tumors, providing a useful addition to their differential diagnosis in difficult cases, and contributing to the understanding of their histogenesis and molecular pathogenesis.

Correspondence to Shotaro Maeda, Department of Pathology, Tama Nagayama Hospital, Nippon Medical School, 1-7-1 Nagayama, Tama-shi, Tokyo 206-8512, Japan
s-maeda@nms.ac.jp

ArticleTitle	Expression and Localization of Basic Fibroblast Growth Factor and its mRNA in Solitary Fibrous Tumor
AuthorList	Xianfeng Li¹, Shotaro Maeda², Masaru Hosone², Hironori Katayama², Namie Sawada¹, Yuliang Sun¹, Toshiyuki Ishiwata¹, Munehiro Yokoyama¹, Zenya Naito¹ and Goro Asano¹
Affiliation	¹Department of Pathology, Nippon Medical School, ²Department of Pathology, Tama Nagayama Hospital, Nippon Medical School
Language	EN
Volume	68
Issue	5
Year	2001
Page	384-392
Received	March 14, 2001
Accepted	April 17, 2001
Keywords	solitary fibrous tumor, basic fibroblast growth factor, immunohistochemistry, in situ hybridization
Abstract	Solitary fibrous tumors (SFTs) represent a distinct neoplasm that should be included in the differential diagnosis of spindle-cell neoplasms of the soft tissue. Basic fibroblast growth factor (bFGF or FGF-2) is a mitogenic and angiogenic polypeptide produced by diverse cell types, including the cells derived from normal tissue and neoplastic lesions. In this study, the expression of bFGF, vimentin, CD 34, c-kit (or CD 117), desmin, S-100 protein, and α-smooth muscle actin (α-SMA) in SFTs, hemangiopericytomas (HPC), gastrointestinal stromal tumors (GIST), and dermatofibrosarcoma protuberans (DFSP) were evaluated to assess their usefulness in the differential diagnosis of these lesions. The expression of bFGF mRNA was also examined in SFTs by in situ hybridization (ISH) using a digoxigenin-labeled bFGF oligonucleotide probe. All the SFTs, GISTs and DFSPs exhibited strong and diffuse immunoreactivity for CD34 and vimentin, and were completely negative for desmin, S-100 protein and α-SMA. The HPCs were positive for vimentin, but negative for CD34. In all the SFTs, strong and diffuse nuclear immunostaining was observed with bFGF antibody, contrasting with the negative staining observed in the majority of the HPCs, GISTs, and DFSPs. The bFGF mRNA was also expressed in the SFT cells. The constitutive expression of the bFGF in the SFT widens the spectrum of available markers for these tumors, providing a useful addition to their differential diagnosis in difficult cases, and contributing to the understanding of their histogenesis and molecular pathogenesis.
Correspondence to	Shotaro Maeda, Department of Pathology, Tama Nagayama Hospital, Nippon Medical School, 1-7-1 Nagayama, Tama-shi, Tokyo 206-8512, Japan s-maeda@nms.ac.jp