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ArticleTitle | Expression of Fibroblast Growth Factor (FGF) -10 in Human Colorectal Adenocarcinoma Cells |
AuthorList | Aihiko Matsuike1, Toshiyuki Ishiwata1, Masanori Watanabe2 and Goro Asano1 |
Affiliation | 1Department of Pathology, Nippon Medical School, 2Center for Digestive Diseases, Second Hospital, Nippon Medical School |
Language | EN |
Volume | 68 |
Issue | 5 |
Year | 2001 |
Page | 397-404 |
Received | February 22, 2001 |
Accepted | April 27, 2001 |
Keywords | FGF-10, colorectal cancer, RT-PCR, in situ hybridization, immunohistochemistry |
Abstract | Fibroblast growth factor (FGF) -10 (keratinocyte growth factor 2, KGF 2) is a new member of the FGF family that is mainly synthesized by mesenchymal cells and acts predominantly on epithelial cells in a paracrine manner. Its actions are dependent on its binding to the iiib isoform of the cell-surface FGF receptor 2 (FGFR2 iiib). FGF-10 is known to play an important role in fetal limb and lung development, skin wound healing and prostatic epithelial cell growth. In the present study, the expression of FGF-10 and FGFR2 iiib in five cultured human colorectal adenocarcinoma cell lines (COLO 205, DLD-1, HCT-15, SW 480 and WiDr) and the localization of FGF-10 messenger RNA (mRNA) and its protein in human colorectal cancer tissues from 10 patients were determined. All five colorectal cancer cell lines expressed FGF-10 mRNA and its protein. FGFR2 iiib mRNAs were expressed in these cells and the recombinant FGF-10 (1ng/ml) increased the growth rate of COLO 205 cells. To determine the localization of FGF-10 protein and its mRNA in normal and cancerous human colorectal tissues, immunohistochemistry and in situ hybridization were performed. In normal colorectal tissues, FGF-10 and its mRNA were not detected. In contrast, moderate immunoreactivity was present in cancer cells in 5 of 10 colorectal cancer cases and mild immunoreactivity was recognized in adjacent fibroblasts. By using in situ hybridization, FGF-10 mRNA was observed in colorectal cancer cells and fibroblasts adjacent to cancer cells. These findings indicate that FGF-10 and its receptor, FGFR2 iiib expression in colorectal adenocarcinoma cells and FGF-10 may contribute to the growth of cells of this type. |
Correspondence to | Toshiyuki Ishiwata, MD, Department of Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan ishiwata@nms.ac.jp |
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