Journal of Nippon Medical School: Vol.69 No.4 page.347

Journal of Nippon Medical School

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ArticleTitle Morphological and Histochemical Characteristics of Mast Cells and the Content of In-tissue Histamine in Various Pathological Parathyroids: Do Mast Cells Participate in Hormone Secretion in Human Parathyroids?

AuthorList Taro Iwamura, Kazuo Shimizu and Shigeo Tanaka

Affiliation Department of Surgery II, Nippon Medical School

Language EN

Volume 69

Issue 4

Year 2002

Page 347-354

Received December 3, 2001

Accepted February 8, 2002

Keywords mast cell, histamine, PTH secretion

Abstract The possibility of the participation of mast cells in human parathyroid hormone secretion was studied with regard to the frequency, distribution, and sub-types of mast cells and the content of in-tissue histamine, a chemical mediator in mast-cell granules, in human parathyroids with various pathological conditions. The above factors were compared between those of a 'normal' parathyroid group and those of 'pathological' parathyroids associated with adenoma and hyperplasia.
Specimens were scanned for the mean value of the mast cell number per field of microscopic view and for the ratio of the mast cell number in glandular parenchymal tissue to that in interstitial tissue. The activated state of the mast cells was examined through classifying the mast cells into two sub-types, mucosal mast cells and connective-tissue mast cells. The high-performance liquid chromatography (HPLC) method was used for assay of in-tissue histamine. The frequency of mast cells showed no difference between the groups, whereas the distribution of mast cells, showed a distinct difference. The occurrence rate of mast cells in glandular parenchymal tissue in the 'pathological' group presented an increase as compared with that in the 'normal' group. Furthermore, the occurrence rate of mucosal mast cells in an activated state also showed an increase. This suggests that mast cells are likely to participate in parathyroid hormone secretion. The histamine-content in the 'normal' group was significantly larger than that in the 'pathological' group, which was a different outcome from that observed in mast cells from the results of light microscopy. This may require taking into consideration the difference in the histamine content of the mast cells themselves between that of mucosal mast cells and connective-tissue mast cells.

Correspondence to Taro Iwamura, MD, Department of Surgery II, Nippon Medical School Second Hospital, 1-396 Kosugicho, Nakahara-ku, Kawasaki-shi, Kanagawa 211-8533, Japan
iwatmr@nms.ac.jp

ArticleTitle	Morphological and Histochemical Characteristics of Mast Cells and the Content of In-tissue Histamine in Various Pathological Parathyroids: Do Mast Cells Participate in Hormone Secretion in Human Parathyroids?
AuthorList	Taro Iwamura, Kazuo Shimizu and Shigeo Tanaka
Affiliation	Department of Surgery II, Nippon Medical School
Language	EN
Volume	69
Issue	4
Year	2002
Page	347-354
Received	December 3, 2001
Accepted	February 8, 2002
Keywords	mast cell, histamine, PTH secretion
Abstract	The possibility of the participation of mast cells in human parathyroid hormone secretion was studied with regard to the frequency, distribution, and sub-types of mast cells and the content of in-tissue histamine, a chemical mediator in mast-cell granules, in human parathyroids with various pathological conditions. The above factors were compared between those of a 'normal' parathyroid group and those of 'pathological' parathyroids associated with adenoma and hyperplasia. Specimens were scanned for the mean value of the mast cell number per field of microscopic view and for the ratio of the mast cell number in glandular parenchymal tissue to that in interstitial tissue. The activated state of the mast cells was examined through classifying the mast cells into two sub-types, mucosal mast cells and connective-tissue mast cells. The high-performance liquid chromatography (HPLC) method was used for assay of in-tissue histamine. The frequency of mast cells showed no difference between the groups, whereas the distribution of mast cells, showed a distinct difference. The occurrence rate of mast cells in glandular parenchymal tissue in the 'pathological' group presented an increase as compared with that in the 'normal' group. Furthermore, the occurrence rate of mucosal mast cells in an activated state also showed an increase. This suggests that mast cells are likely to participate in parathyroid hormone secretion. The histamine-content in the 'normal' group was significantly larger than that in the 'pathological' group, which was a different outcome from that observed in mast cells from the results of light microscopy. This may require taking into consideration the difference in the histamine content of the mast cells themselves between that of mucosal mast cells and connective-tissue mast cells.
Correspondence to	Taro Iwamura, MD, Department of Surgery II, Nippon Medical School Second Hospital, 1-396 Kosugicho, Nakahara-ku, Kawasaki-shi, Kanagawa 211-8533, Japan iwatmr@nms.ac.jp