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ArticleTitle | Mutational Analysis of the Macrophage Scavenger Receptor 1 (MSR1) Gene in Primary Lung Cancer |
AuthorList | Akinobu Yoshimura1, Akihiko Gemma1, Kiyoko Kataoka1, Yoko Hosoya1, Rintaroh Noro1, Masahiro Seike1, Yutaka Kokubo1, Masatoshi Watanabe2 and Shoji Kudoh1 |
Affiliation | 1Fourth Department of Internal Medicine, Nippon Medical School 2Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University |
Language | EN |
Volume | 71 |
Issue | 2 |
Year | 2004 |
Page | 99-104 |
Received | August 1, 2003 |
Accepted | October21, 2003 |
Keywords | lung cancer, deletion, macrophage scavenger receptor 1 (MSR1) gene, mutation, polymorphism |
Abstract | Allelic deletion at chromosome 8p21-25 is an early and frequent event in the carcinogenesis and development of various cancers. To facilitate investigation of alterations of the macrophage scavenger receptor 1 (MSR1), which is located on 8p22, and to determine the role of this gene in human carcinogenesis and tumor progression, we determined intronic primers designed to amplify the coding region. Since frequent deletion of 8p21-23 has been previously reported in lung cancer, we searched for mutations throughout the coding sequence of the MSR1 gene within a panel of genomic DNA samples obtained from 30 primary lung cancers. Our approach, which involved polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis and direct DNA sequencing, revealed nucleotide variants of the MSR1 gene in only one of the 30 cases examined, with this sample displaying both a 6 bp deletion and a thymine-to-cytosine substitution, the latter occurring within intron 7. The 6 bp deletion was located at a DNA microsatellite region and the thymine-to-cytosine substitution seemed to be a polymorphism. |
Correspondence to | Correspondence to Shoji Kudoh, Fourth Department of Internal Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan kuntonjp@nms.ac.jp |
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