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ArticleTitle | Effect of Ionizing Irradiation on Human Esophageal Cancer Cell Lines by cDNA Microarray Gene Expression Analysis |
AuthorList | Hideki Bo1,2, Mohammad Ghazizadeh1, Hajime Shimizu1, Yuji Kurihara2, Seiko Egawa1, Yukichi Moriyama2, Takashi Tajiri3, Oichi Kawanami1 |
Affiliation | 1Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School 2Center for Digestive Diseases, Nippon Medical School Second Hospital 3Surgery for Organ Function and Biological Regulation, Nippon Medical School, Graduate School of Medicine |
Language | EN |
Volume | 71 |
Issue | 3 |
Year | 2004 |
Page | 172-180 |
Received | January 9, 2004 |
Accepted | January 19, 2004 |
Keywords | esophageal squamous cell carcinoma (ESCC), cell line, radiation, gene expression, cDNA microarray |
Abstract | To provide new insights into the molecular mechanisms underlying the effect of irradiation on esophageal squamous cell carcinomas (ESCCs), we used a cDNA microarray screening of more than 4,000 genes with known functions to identify genes involved in the early response to ionizing irradiation. Two human ESCC cell lines, one each of well (TE-1) and poorly (TE-2) differentiated phenotypes were screened. Subconfluent cells of each phenotype were treated with single doses of 2.0 Gy or 8.0 Gy irradiations. After a 15 min incubation time-point, the cells were collected and analyzed. Compared with non-irradiated cells, many genes revealed at least 2-fold upregulation or downregulation at both doses in well or poorly differentiated ESCC cells. The common upregulated genes in well and poorly differentiated cell types at both irradiation doses included SCYA5, CYP51, SMARCD2, COX6C, MAPK8, FOS, UBE2M, RPL6, PDGFRL, TRAF2, TNFAIP6, ITGB4, GSTM3, and SP3 and common downregulated genes involved NFIL3, SMARCA2, CAPZA1, MetAP2, CITED2, DAP3, MGAT2, ATRX, CIAO1, and STAT6. Several of these genes were novel and not previously known to be associated with irradiation. Functional annotations of the modulated genes suggested that at the molecular level, irradiation appears to induce a regularizing balance in ESCC cell function. The genes modulated in the early response to irradiation may be useful in our understanding of the molecular basis of radiotherapy and in developing strategies to augment its effect or establish novel less hazardous alternative adjuvant therapies. |
Correspondence to | Mohammad Ghazizadeh, MD, Ph D, Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School, 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, 211-8533, Japan ciem@nms.ac.jp |
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