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ArticleTitle “]ˆÚ”\‚ĖˆŲ‚Č‚éƒqƒg”x‘BŠāŠ”‚É‚¨‚¯‚錌ŠĮVļ”\‚ĖˇˆŲ
AuthorList įž ‘哯1, a’J š•F2, ŽÂ“c ‹Ķ–į3, “ú”ä–ė r1, ŧ“c ‹vmŽq1, ’|’† Œ\1, ŒˇŠÔ ē•F1, H“Ą ãēņ1
Affiliation 1“ú–{ˆã‰Č‘åŠw“ā‰ČŠw‘æ4‹ŗŽē
2“Œ‹ž“s—§‹îž•a‰@ŒÄ‹zŠí“ā‰Č
3‘—§‘Ûˆã—ÃZƒ“ƒ^[ŒÄ‹zŠí‰Č
Language JA
Volume 71
Issue 3
Year 2004
Page 181-189
Received August 8, 2003
Accepted February 9, 2004
Keywords human lung adenocarcinoma cell line, angiogenesis, metastasis, VEGF, MMP-2
Abstract We investigated the ability of angiogenesis in PC9/F9 cells (from a highly metastatic human lung adenocarcinoma cell line) as compared with PC9 cells (from a low metastatic human lung adenocarcinoma cell line). In vivo tumor growth assay using BALB/c nude mice (7 mice/group), showed that the tumor volume of PC9/F9 cells on day 35 (230.7±31.3 mm3) was significantly larger than that of PC9 cells (90.9±24.7 mm3) (p<0.001). However, there was no significant difference between PC9/F9 cells and PC9 cells in an in vitro growth assay. In a dorsal air sac assay (DAS assay) using ICR mice (3 mice/group), PC9/F9 cells (4.7±1.2 vessels) showed stronger neovascurizationin in compared with PC9 cells (0.3±0.4 vessels) (p<0.05). In an enzyme linked immunosorbent assay (ELISA) and Western blotting analysis there were no significant differences between PC9/F9 cells and PC9 cells in the protein expression of vascular endothelial growth factor (VEGF). There was no significant difference between the gene expression levels of PC9/F9 cells and PC9 cells on cDNA array analysis. Matrix metalloproteinase-2 (MMP-2) activity in PC9/F9 cells was remarkably stronger than that of PC9 cells in Gelatin Zymography.
From these results, we considered that of the increased metastasis of PC9/F9 cells might be induced by augmented angiogenesis. Furthermore, we speculated that the augmented angiogenesis of the highly metastatic PC9/F9 cell line might be induced by increased MMP-2 activity.
Correspondence to Datong Zou, MD, Fourth Department of Internal Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan
zoudatong@hotmail.com

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