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Imaging Mitochondrial Redox Environment and Oxidative Stress Using a Redox-Sensitive Fluorescent Protein

Alexander M. Wolf, Sadamitsu Asoh, Ikuroh Ohsawa and Shigeo Ohta

Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Graduate School of Medicine, Nippon Medical School

Redox-sensitive green fluorescent protein (roGFP) is a fluorescent protein in which two cysteines are placed adjacently in the barrel structure. Disulfide formation (oxidation) increases the absorption at short wavelengths (410 nm) at the expense of absorption at longer wavelengths (490 nm). The fluorescence ratio indicates reduction/oxidation, i.e., the redox potential at specific cellular locations.

J Nippon Med Sch 2008; 75: 66-67

Correspondence to
Sadamitsu Asoh, Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Graduate School of Medicine, Nippon Medical School, 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211-8533, Japan
sada@nms.ac.jp