-Short Communication-

Success Rate of Collagen Gel Droplet-embedded Culture Drug Sensitivity Test in Colorectal Cancer: Are Antibiotics a Prerequisite for Specimen Irrigation?

Takeshi Yamada¹, Hayato Kan¹, Satoshi Matsumoto¹, Yoshikazu Kanazawa¹, Michihiro Koizumi¹, Junpei Sasaki¹, Aya Tani¹, Kimiyoshi Yokoi^1,2 and Eiji Uchida¹

¹Surgery for Organ Function and Biological Regulation, Graduate School of Medicine, Nippon Medical School
²Department of Surgery, Nippon Medical School Chiba Hokusoh Hospital

The collagen gel droplet-embedded culture drug sensitivity test (CD-DST) is one of the best chemosensitivity tests owing to its high success rate. However, CD-DST is often a culture method, and contamination is a serious problem, especially in the case of colorectal cancer, which is contaminated by enteric bacteria. It has been reported that the success rate of CD-DST is 64.0% in the case of colorectal cancer. Therefore, the sampling and washing of specimens before culture are extremely important. By washing specimens carefully with normal saline containing antibiotics, we achieved a success rate of 85.3% in the case of colorectal cancer. To improve the success rate, we started specimen irrigation with a large amount of normal saline in January 2007. As a result, a success rate exceeding 90% was acquired. For the success of CD-DST for colorectal cancer, it is important to irrigate specimens many times with a large amount of normal saline.

J Nippon Med Sch 2012; 79: 163-165

Keywords
collagen gel droplet-embedded culture drug sensitivity test, colon cancer, chemosensitivity test

Correspondence to
Takeshi Yamada, Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan
y-tak@nms.ac.jp

Received, September 7, 2011
Accepted, February 24, 2012