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Plasma Adsorption Membranes Are Able to Efficiently Remove High Mobility Group Box-1 (HMGB-1)
1The Department of Emergency and Critical Care, Ureshino Medical Center, Saga, Japan
2The Department of Emergency and Critical Care Medicine, Saga University Hospital, Saga, Japan
3The Division of Trauma Surgery and Surgical Critical Care, Saga University Hospital, Saga, Japan
Background: High Mobility Group Box 1 (HMGB-1) is a 30 kDa protein that is a lethal mediator in sepsis and is a recognized therapeutic target. However, no consensus has been reached for acute blood purification therapy as a treatment for sepsis targeting HMGB-1. Previous studies demonstrated that a high anti-HMGB-1 antibody titer and the suppression of HMGB-1 activity improved survival rate in animal sepsis models. The aim of this study was to evaluate whether plasma adsorption therapy is able to decrease the level of HMGB-1, representing a new potential treatment strategy against sepsis.
Methods: Plasma adsorption therapy has been known as a treatment for various autoimmune diseases. Three different adsorbent columns, including TR-350 (IM-TR), PH-350 (IM-PH), and BRS-350 (BRS), were used in this study for comparison.
We made a 1/350 scale of these three columns. Fetal bovine serum (FBS) contains a significant amount of HMGB-1. After priming with saline, FBS was passed through the columns and the adsorption rates of HMGB-1 at 25 minutes, 50 minutes, and 75 minutes were evaluated. The total adsorbed HMGB-1 level at 75 minutes was also calculated.
Results: The highest adsorption rate and total adsorbed amount of HMGB-1 were observed in IM-TR, followed by BRS and IM-PH. IM-TR showed a decline in adsorption rate over time. BRS showed a steady adsorption rate at all time points. IM-TR removed HMGB-1 significantly more than IM-PH and BRS.
Conclusions: Our findings showed that plasma adsorption therapy efficiently adsorbed HMGB-1 and could be safely applied for the treatment of sepsis.
J Nippon Med Sch 2018; 85: 150-156
Keywords
HMGB-1, sepsis, plasma adsorption, charge, hydrophobicity
Correspondence to
Takayuki Onohara, The Department of Emergency and Critical Care, Ureshino Medical Center, 2436 Shimojuku Ureshino, Saga 843-0393, Japan
m05019to@jichi.ac.jp
Received, December 13, 2017
Accepted, January 12, 2018