-Original-

Oxidative Stress and Antioxidant Capacity in Patients with Endometrioma

Go Ichikawa^1,2, Yasuyuki Negishi^1,3, Ryo Tsuchiya¹, Lilika Higuchi¹, Tatsunori Shiraishi¹, Mariko Ikeda¹, Hanako Kaseki^1,2, Rimpei Morita³ and Shunji Suzuki¹

¹Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan
²Department of Obstetrics and Gynecology, Nippon Medical School Chiba Hokusoh Hospital, Chiba, Japan
³Department of Microbiology and Immunology, Nippon Medical School, Tokyo, Japan

Background: Endometriosis has several clinical features, including dysmenorrhea, infertility, and endometrioma (EMO). Although oxidative stress status is closely related to endometriosis, it is unclear how the balance between oxidative stress capacity and antioxidant capacity correlates with treatment of or factors that worsen endometriosis. In this study, we used peritoneal fluid from patients with EMO to investigate the role of oxidative stress capacity and antioxidant capacity.
Materials and Methods: Participants with EMO (n = 30) and without EMO (uterine myoma, n = 13) were enrolled. All peritoneal fluid samples were collected at the beginning of surgery. We evaluated oxidative stress capacity and antioxidant capacity in peritoneal fluid samples by using the diacron-reactive oxygen metabolites (d-ROM) and biological antioxidant potential (BAP) tests, respectively. The d-ROM and BAP values and the d-ROM/BAP ratio were measured, and their correlations with the CA125 level, revised American Society for Reproductive Medicine (r-ASRM) score, and tumor size were analyzed.
Results: The d-ROM/BAP ratio was significantly higher in patients with EMO than in those without EMO. In addition, the d-ROM/BAP ratio was positively correlated with CA125 level and r-ASRM scores in patients with EMO.
Conclusions: Oxidative stress is correlated with factors that worsen EMO. The d-ROM/BAP test may be useful for assessing disease status in patients with EMO.

J Nippon Med Sch 2024; 91: 146-154

Keywords
endometriosis, endometrioma, oxidative stress

Correspondence to
Yasuyuki Negishi, MD, PhD, Department of Microbiology and Immunology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
negi@nms.ac.jp

Received, July 25, 2023
Accepted, October 4, 2023