Journal of Nippon Medical School: Vol.67 No.2 page.092

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ArticleTitle Evaluation of Glycosaminoglycans Levels in Normal Joint Fluid of the Knee

AuthorList Yoshihito Nakayama, Yasumasa Shirai, Kiyoshi Yoshihara and Shinji Uesaka

Affiliation Department of Orthopaedic Surgery, Nippon Medical School

Language EN

Volume 67

Issue 2

Year 2000

Page 92-95

Received Received, November 8, 1999

Accepted Accepted, December 8, 1999

Keywords hyaluronic acid, chondroitin sulfate, normal joint fluid, biochemical analysis

Abstract Glycosaminoglycans (GAGs) level in joint fluid have been investigated in various joint diseases as a joint maker. However, there are very few studies of normal joint fluid providing a baseline for a better understanding of altered GAGs level in pathological joint fluid. We investigated GAGs level in knee joint fluid for 25 healthy young volunteers with a mean age of 27.5 years (range: 18 to 36 years) . Biochemical evaluations included the concentration of hyaluronic acid (HA) , chondroitin 4-sulfate (C4S) and chondroitin 6-sulfate (C6S) , and the C6S/C4S ratio. The unsaturated disaccharides derived from HA by Morgan-Elson methods, and those from CS were measured with high performance liquid chromatography. The mean HA concentration was 3.4 ± 0.6 mg/ml. The mean concentration of C4S and C6S, and C6S/C4S ratio were 19.0 ± 4.9 nmol/ml, 125.5 ± 44.4 nmol/ml and 6.5 ± 1.1 respectively.

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ArticleTitle	Evaluation of Glycosaminoglycans Levels in Normal Joint Fluid of the Knee
AuthorList	Yoshihito Nakayama, Yasumasa Shirai, Kiyoshi Yoshihara and Shinji Uesaka
Affiliation	Department of Orthopaedic Surgery, Nippon Medical School
Language	EN
Volume	67
Issue	2
Year	2000
Page	92-95
Received	Received, November 8, 1999
Accepted	Accepted, December 8, 1999
Keywords	hyaluronic acid, chondroitin sulfate, normal joint fluid, biochemical analysis
Abstract	Glycosaminoglycans (GAGs) level in joint fluid have been investigated in various joint diseases as a joint maker. However, there are very few studies of normal joint fluid providing a baseline for a better understanding of altered GAGs level in pathological joint fluid. We investigated GAGs level in knee joint fluid for 25 healthy young volunteers with a mean age of 27.5 years (range: 18 to 36 years) . Biochemical evaluations included the concentration of hyaluronic acid (HA) , chondroitin 4-sulfate (C4S) and chondroitin 6-sulfate (C6S) , and the C6S/C4S ratio. The unsaturated disaccharides derived from HA by Morgan-Elson methods, and those from CS were measured with high performance liquid chromatography. The mean HA concentration was 3.4 ± 0.6 mg/ml. The mean concentration of C4S and C6S, and C6S/C4S ratio were 19.0 ± 4.9 nmol/ml, 125.5 ± 44.4 nmol/ml and 6.5 ± 1.1 respectively.
Correspondence to