ArticleTitle |
Evaluation of Glycosaminoglycans Levels in Normal Joint Fluid of the Knee |
AuthorList |
Yoshihito Nakayama, Yasumasa Shirai, Kiyoshi Yoshihara and Shinji Uesaka |
Affiliation |
Department of Orthopaedic Surgery, Nippon Medical School |
Language |
EN |
Volume |
67 |
Issue |
2 |
Year |
2000 |
Page |
92-95 |
Received |
Received, November 8, 1999 |
Accepted |
Accepted, December 8, 1999 |
Keywords |
hyaluronic acid, chondroitin sulfate, normal joint fluid, biochemical analysis |
Abstract |
Glycosaminoglycans (GAGs) level in joint fluid have been investigated in various joint diseases as a joint maker. However, there are very few studies of normal joint fluid providing a baseline for a better understanding of altered GAGs level in pathological joint fluid. We investigated GAGs level in knee joint fluid for 25 healthy young volunteers with a mean age of 27.5 years (range: 18 to 36 years) . Biochemical evaluations included the concentration of hyaluronic acid (HA) , chondroitin 4-sulfate (C4S) and chondroitin 6-sulfate (C6S) , and the C6S/C4S ratio. The unsaturated disaccharides derived from HA by Morgan-Elson methods, and those from CS were measured with high performance liquid chromatography. The mean HA concentration was 3.4 ± 0.6 mg/ml. The mean concentration of C4S and C6S, and C6S/C4S ratio were 19.0 ± 4.9 nmol/ml, 125.5 ± 44.4 nmol/ml and 6.5 ± 1.1 respectively. |
Correspondence to |
@ |